Document Type : Research Paper
Department of Pathology, School of Medicine, Gastrointestinal Cancer Research Center, Non-Communicable Diseases Research Institutes Mazandaran University of Medical Sciences, Sari, Iran.
Department of Internal Medicine, School of Medicine, Gut and Liver Research Center, Non-communicable Diseases Research Institutes, Mazandaran University of Medical Sciences, Sari, Iran.
Research Center for Liver Diseases, Keck School of Medicine, Department of Medicine, University of Southern California, Los Angeles, CA, USA.
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.
Background and Objective: A large number of KRAS mutations in codons 12 and 13 exons two have been reported in gastric cancer (GC). This study aimed to investigate the frequency of codon 12 and 13 mutations in the KRAS gene in patients with gastric cancer in northern Iran.
Methods: This is a descriptive cross-sectional study that was performed on patients referred for an endoscopy to Imam Khomeini Hospital in Sari, from 2009-2015. The sample size was assumed to be 100 people. Of the 100, 50 were isolated with a healthy tissue sample and placed in a group based on endoscopic results. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to evaluate codons 12 and 13 of KRAS gene located in Exon No. 1 of this gene. Statistical tests were performed using SPSS version 24.
Results: In this study, for both codon 12 and codon 13, in cancerous tissue samples, the results were normal and no mutations in the KRAS gene were found. Also, in the normal samples obtained from the patients, the study of codon 12 and 13 in the KRAS gene did not show any change. No mutations in nucleotide G in KRAS gene were found in patients.
Conclusions: The results of the present study showed that there was no association between codon 12 and 13 mutations in the KRAS gene with GC.