Antagonistic Effect of Probiotics on Drug Resistant Pseudomonas aeruginosa Isolated From Burn Wound Infection

Document Type : Research Paper


Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran



Background and Objectives: Pseudomonas aeruginosa is an opportunistic bacterium and is one of the main causes of infection in burn patients. As this bacterium gets resistant to various types of antibiotics through different acquiring mechanisms, it can easily induce skin deformities and even patients’ death.
Methods: This descriptive study was carried out on 200 patients with burn injuries hospitalized in specialized burn hospitals. Culture methods and diagnosis tests were used to separate and diagnose P. aeruginosa, and disk diffusion Agar method with the Kirby-Bauer standard was applied to determine the pattern of drug-resistance. The antagonist effect of lactic bacteria isolated from 14 samples of milk and yogurt on the growth of P. aeruginosa was also assessed using Agar well diffusion method.
Findings: Out of 30 strains diagnosed and isolated as P. aeruginosa 45.2% were resistant to gentamicin, 51.6% to cefotaxime, 48.8 to imipenem, and 45.2 to ciprofloxacin. Study of probiotics revealed that Lactobacillus casei, with the average diameter of 20.3 mm, has the highest inhibitory effect against P. aeruginosa.
Conclusions: The results of this study showed that the spread of P. aeruginosa resistant to medicine is very high in the surveyed hospitals, and that the isolated lactobacilli have a significant inhibitory effect on drug resistant P. aeruginosa strains. Our results hence suggest that, using probiotic products would help better control of P. aeruginosa infection in burn patients.